Oxalis corniculata

cbd_2014_pathway:escape_horticulture

Perennial herb

Europe and North Africa Europe has five extant species of Oxalis section Corniculatae, O. corniculata, O. exilis, O. stricta, O. dillenii and O. filiformis Kunth (syn. O. ferae L. Llorens, Gil & C. Cardona). Of these, O. exilis and O. filiformis are 20 th century introductions with relatively narrow distributions (Llorens et al., 2005; Young, 1968). Owing to the presence of closely related species in Europe it is necessary to consider confusion of their identification in observations. Unless a complete specimen is available it is difficult to distinguish these taxa from one another, particularly if only fragments, such as seeds, are available. Taxonomic and nomenclatural confusion is a constant theme in the literature of this section (Eiten, 1955; Watson, 1989). Therefore, before investigating the situation of O. corniculata it is important to establish the time of introduction of O. stricta and O. dillenii to Europe. It was not until 1680 that Morison described a species with an upright habit that we now refer to as O. stricta (Morison, 1680). However, this upright species was described from seed sent to him from Virginia and was not from Europe. By the mid- 19 th century the upright species (O. stricta) was so well established in Europe that Jordan (1854) considered that it must be a native species and described O. europaea Jord. He did so based purely on the geographic grounds that the Linnaean O. stricta was North American. He did not give any morphological characters that separate them. Herbarium specimens do exist of O. stricta collected in Europe before 1854, but these are few and only date from earlier in the 19 th century (Table 1) (Lourteig, 1979). O. dillenii was described and illustrated by Dillenius (1732) and was formally named later by Jacquin (1794) with direct reference to Dillenius. However, this was from material of North American origin. It was not until later that O. dillenii was recognised as naturalized in Europe. Perhaps the first record is that of Jordan (1854) who named O. navieri Jord., which is now considered a synonym for O. dillenii. Therefore, we agree with all modern authors that both O. stricta and O. dillenii are 19 th century introductions and we can assume that earlier European observations of caulescent yellow-flowered Oxalis are of O. corniculata. The earliest clear mention of a yellow-flowered caulescent Oxalis in European literature is from northern Italy in the 16 th century (Turner, 1568). The earliest herbarium specimens are also from northern Italy, from Florence in 1600 and Bologna between 1551 and 1600 (Table 1). Watson (1989) reviewed the taxonomic situation of O. corniculata in Europe and cites Renaissance botanists of the 16 th and 17 th century from Belgium, France, Germany, the Netherlands, Spain and Switzerland. Therefore, there is good evidence, including accurate drawings, that O. corniculata occurred in Europe at least from the 16 th century. Earlier than this the information in literature is ambiguous. We have found six illustrations of Oxalis (Alleluia or Panis Cuculi in Latin) in medieval herbals from the 13 th to 15 th centuries. In each case only one taxa is illustrated. In Europe we would expect at least one taxon, the native O. acetosella L. This species is quite distinct from O. corniculata in having white flowers, short globular pods, a scaly creeping rhizome and no stem. The illustration in the 15 th century Codex Bellunensis and an unnamed codex have no flowers, but depict the scaly rhizome and the lack of stem, which suggests this is O. acetosella (British Library, 2019 a; Schoenberg Center, 2019). The illustration in Giovanni Cadamosto’ s manuscript of the late 15 th or early 16 th century ambiguously shows a part caulescent and part acaulescent plant, (British Library, 2019 b). The flowers of this illustration are in bud and are of little diagnostic use. A similarly ambiguous picture is in a codex dated 1475 – 1525, also from Italy (University of Vermont Libraries, 2019). Another herbal from circa 1440 shows a caulescent Oxalis with small yellow flowers in umbels. This illustration is close to the characters of O. corniculata and originates from Lombardy in northern Italy (British Library, 2019 c). Finally, the oldest illustration is in the Tractatus de herbis (circa 1280 – 1350) (British Library, 2019 d). This is a rather confusing illustration. It shows a horizontal creeping plant, rooting at the nodes, which could be O. acetosella or O. corniculata. The presumed flowers are small and erect, like O. corniculata, but solitary like those of O. acetosella. There are other four-pointed structures, which are presumably fruits. However, they do not resemble Oxalis fruits. A considerable number of archaeobotanical studies have been conducted in Europe, but remains of Oxalis section Corniculatae remain rare. The RADAR database in the Netherlands contains four records, the earliest of which are from a site that spans the 17 th and 18 th centuries (Van Haaster & Brinkkemper, 1995). In Ferrara, Italy, remains of a single seed were found in a waste pit dating from the second half of the 15 th century, which is notable because of the pre-Columbian dating (Bosi, 2000, Bosi et al., 2009). Although a single seed, its location is close to the specimen, illustration and literature reports from the 15 th and 16 th centuries, giving credence to this isolated observation. We have found no earlier archaeological evidence, even though some extensive studies have been conducted. Rinaldi, Mazzanti & Bosi (2013) summarize the seeds found in excavations in Modena, in northern Italy from the 2 nd century BC to the 6 th century AD. They list more than 400 taxa from 200,000 remains, including many weeds, but did not find O. corniculata. Likewise a similar study in Lleida, Spain, covering the period between the 2 nd century BC and the 11 th century AD report no O. corniculata, despite recovering almost 600,000 seeds and fruits. We have found two other archaeological reports of O. corniculata in Europe, one from Neolithic Germany in the 6 th century BC and the other from Visigothic Spain in the 6 th- 7 th century AD (Herbig et al., 2013; Ollich et al., 2014). The reports are only of two and one seed, respectively, and there are no illustrations. Given the small number of seeds, the lack of supporting evidence and the potential for later contamination or misidentification, we have discounted these reports. In all, we found no convincing evidence for the presence of O. corniculata in Europe earlier than the 15 th century. Neither is there much information from North Africa, which has been in constant communication with southern Europe for many centuries. Preserved Oxalis seeds have been reported from late neolithic Hyrax middens in the Hoggar Mountains in the Sahara (Barakat, 1995). However, no illustration is provided and there is no way to verify this unlikely claim. A more reliable source is from excavations at ancient Carthage in modern day Tunisia (Van Zeist, Bottema & Van Der Veen, 2001). Here, preserved Oxalis seeds were reported from sediment from approximately the 4 th century BC to the mid- 6 th century AD. However, there is again no illustration and we have been unable to access vouchers. The earliest direct observation from North Africa is from 1751 (Hasselquist, 1766). This was near Damietta, Egypt, in the Nile delta. In conclusion, there is good evidence of O. corniculata being in Europe from the 16 th century onward. Despite considerable taxonomic confusion, there is no evidence of O. stricta and O. dillenii occurring here much before the beginning of the 19 th century, presumably as an introduction from North America, as is generally assumed. Earlier than this, despite a copious corpus of botanical, medicinal and archaeobotanical literature, the evidence is weak, leaving an open question about the long-term existence of O. corniculata in Europe. The observation of O. corniculata in Carthage may indicate that O. corniculata was present in the Mediterranean basin during the Iron Age, though more evidence than this would be preferable. Therefore there is little support for O. corniculata being native to Europe. Sub-Saharan Africa Today O. corniculata is a common weed in sub-Saharan Africa (Holm et al., 1991). However, Hooker & Bentham (1849) specifically noted its absence from the western intertropical coast, where it is present today (Hutchinson & Dalziel, 1954; Holm et al., 1991). The earliest sub-Saharan records are of Thunberg from near Cape Town, South Africa, circa 1772 (Table 1). Thunberg described its habitat as “ Crescit prope urbem locis aquosis et in hortis ” (Growing in wet ground near the city gardens) (Thunberg, 1823). Thunberg described O. repens Thunb. from these specimens, which has subsequently been synonymized with O. corniculata (Lourteig, 1979). The next specimens are from the 1830 s from South Africa and Ethiopia (Table 1), but there are few records from sub-Saharan Africa until the 20 th century and many records are of O. corniculata as an agricultural weed. In Ethiopia Richard, Petit & Quartin-Dillon (1847) claimed three species, O. corniculata and two new species O. procumbens and O. radicosa. Lourteig (1979) accepted O. procumbens and O. radicosa as distinct species. Oxalis procumbens is said to grow in fields and fallow land at altitudes above 1,000 m in tropical Africa and O. radicosa has a larger distribution spanning much of Africa, Asia and Australasia. Examples of specimens are in Table 1. Many other authors, at least in Africa, have treated these species as synonyms of O. corniculata (Engler, 1894; Madagascar Catalogue, 2017). If one accepts that O. procumbens and O. radicosa are distinct species in Africa, then there is little reason to think that O. corniculata s. s. is native to sub-Saharan Africa. However, if these species are considered to be part of O. corniculata s. s. then it is perhaps native to the east coast of Africa and Madagascar and also to high altitude areas of tropical Africa. However, the lack of systematic collecting in Africa before the 20 th century means that the indigenousness of specimens attributed to O. procumbens and O. radicosa can also not be confirmed. The lack of data from much of Africa make it difficult to draw conclusions and this is further complicated by the ambiguity of the species concept of O. corniculata. The history of O. corniculata in sub-Saharan Africa will probably remain obscure without further evidence or discoveries. Australasia Australasia has several native species in section Corniculatae, but O. corniculata s. s. is considered introduced (Webb, Sykes & Garnock-Jones, 1988; Conn, Jeanes & Richards, 1999; Gray, 2011). Native taxa are O. novae-caledoniae R. Knuth & Schltr., O. exilis, O. rubens Haw, O. perennans Haw., O. chnoodes Lourteig, O. radicosa and O. thompsoniae B. J. Conn & P. G. Richards. Unlike authors of African Floras, authors of Australasian Floras accept O. radicosa as a distinct species. Australasian species live in a wide range of natural and anthropogenic habitats and although all are close in morphology to O. corniculata, all have distinct morphological features that separate them. Nevertheless, confusion of specimens and observations of O. corniculata with the native taxa must always be considered. Study of the Australasia situation is complicated because Eiten considered all Australasian members of the section Corniculatae to be part of O. corniculata s. l. and many specimens in herbaria retain his determinations (Eiten, 1963). Of possible native species, the closest species to O. corniculata in Australasia is O. thompsoniae that has recently been described (Conn & Richards, 1994). It has a weedy habitat and is only known from collections made from the late 20 th century. Conn & Richards (1994) suggest it may have been introduced to Australasia, but is of unknown origin. Likewise it may have evolved recently in Australasia from O. corniculata. Nevertheless, all reliable determinations of O. corniculata s. s. in Australasia date from the mid to late 20 th century. In summary, the prevailing consensus that O. corniculata is a recent introduction to Australasia seems to be consistent with the available evidence. However, the similarities of Australasian species with O. corniculata perhaps indicate a close evolutionary relationship. North America The Flora of North America lists several native members of Oxalis section Corniculatae. These are O. illinoensis Schwegman, O. macrantha (Trel.) Small, O. grandis Small, O. colorea (Small) Fedde, O. stricta, O. suksdorfii Trel., O. dillenii, O. albicans Kunth, O. pilosa Nutt. ex Torr. & A. Gray, O. californica (Abrams) R. Knuth and O. texana (Small) Fedde. Furthermore, in Mexico, Central America and the Caribbean there are other species, O. filiformis, O. rugeliana Urb. and O. thelyoxys Focke (Nesom, 2017). Oxalis corniculata s. s. is considered an introduction to North America (Nesom, 2009). In Central America and the Caribbean the earliest herbarium specimens date from the 1820 s (Lourteig, 1979). However, an illustration in Hernández (1651) has been interpreted as being O. corniculata (Pico & Nuez, 2000), although the illustrated specimen cannot be confidently distinguished from other North American taxa, such as O. albicans. We found no convincing evidence that conflicts with the widely held view that O. corniculata has been introduced to North America. South America Several species in section Corniculatae have been reported in South America such as O. bisfracta Turcz., O. calachaccensis R. Knuth, O. corniculata, O. filiformis and O. sexenata Savigny (Eiten, 1963). Also, others, such as O. dumetorum Barnéoud and O. conorrhiza Jacq. can be included under a broader circumscription of section Corniculatae (Vaio et al., 2013). Vaio et al. (2013) used a molecular phylogeny to trace the evolutionary roots of section Corniculatae to South America. However, establishing whether O. corniculata s. s. is native to South America is complicated because of the other closely related species present on the continent. Where observations are not supported by specimens it is impossible to know if they have been correctly identified. The earliest specimens we have found of O. corniculata from South America (Brazil) are from 1815 to the 1830 s (Table 1). However, in both cases the plants were either cultivated or weeds of cultivation. Vaio et al. (2013) showed that O. corniculata is genetically close to the Bolivian species O. calachaccensis. In fact, O. calachaccensis does not possess any character that uniquely distinguishes it from O. corniculata (Eiten, 1963). O. calachaccensis was described by Knuth (1915) using material he collected in 1911 from La Paz, Bolivia and one is left to assume it is native to that location. However, as Vaio et al. (2013) indicate, the taxonomic status of O. calachaccensis is doubtful. No earlier literature or archaeobotanical evidence has been found for O. corniculata s. s in South America. Even though O. corniculata has South American ancestry, there is no evidence for the species being in South America before the 19 th century. Asia Oxalis corniculata is widespread in Asia and is an important weed in countries such as India and China (Reddy, 2008; Liu & Watson, 2008). The authors of Asian Floras, such as those from China and Japan, do not commit to whether O. corniculata is native or introduced, but they conclude that the widespread dispersal by humans has made its origin obscure (Ōi, Meyer & Walker, 1965; Liu & Watson, 2008). Nevertheless, there are several archaeobotanical reports of O. corniculata seed from China. The earliest of these are from the Majiabang culture (Hunan, 7000 – 5800 BP; 16 seeds in two excavations) and Daxi culture (Jiangsu, 7,000 – 5,300 BP; 17 seeds) (Nasu et al., 2012; Qiu et al., 2016). Then are reports from Henan Province 4,950 – 4,450 BP where 74 seeds were found (Deng et al., 2015). There are also reports of multiple preserved seed from the Huangguashan period in Fujian province from more than 3,000 BP; from the Bronze Age in Yunnan province, and from during the Tang dynasty in Lantau (Atha, 2012; Yao et al., 2015; Deng et al., 2017). The paper of Nasu et al. (2012) has a particularly convincing illustration of a seed. At all of these sites there was evidence of early agriculture where O. corniculata may have been a weed. Apart from O. corniculata the only other possibility is that these are seeds of O. stricta, which is believed to be native to China (Liu & Watson, 2008). However, this Flora of China describes O. stricta as growing in “ Forests, ravines; 400 – 1,500 m ” in Guangxi, Hebei, Henan, Hubei, Jiangxi, Jilin, Liaoning, Shanxi, Zhejiang. This is not consistent with the presence of this Oxalis in either Hunan, Fujian, Yunnan or Jiangsu province as an agricultural weed. Also in Asia, Oxalis seeds have been found in Japan from the Early Yayoi period (ca. 2,820 – 2,530 BP); in the Philippines in the 1 st millennium BC and in Vietnam between 3,450 and 3,250 BP (Paz, 2005; Nasu & Momohara, 2016; Castillo et al., 2018). Castillo et al. (2018) provide a photograph of the single seed they found. Nasu & Momohara (2016) indicate that they found between 11 and 58 seeds across two different excavations. O. stricta has neither been found in the Philippines nor Vietnam, so it seems reasonable to assume these are seeds of O. corniculata. There is early documentary evidence of the presence of O. corniculata in China from 1751 (Osbeck, Torén & Ekeberg, 1771). This is from Guangdong, outside the known range of O. stricta in China and in a region where O. corniculata is common today. There is also a specimen from Osbeck in the Bergius Herbarium from 1751 and this has been identified by Lourteig as O. corniculata (Table 1). The distribution of first records illustrated in Fig. 1 demonstrates the cluster of early records from south – east Asia. Oxalis corniculata is also the main food plant of the larvae of Pseudozizeeria maha Kollar, 1844 (pale grass blue butterfly) (Saji et al., 2017). This species is found across India, south – east Asia, China, North and South Korea and Japan. The presence of this largely monophagous insect suggests that the original native range of its host plant lies within Asia. While it is an indirect indication, the use of plants in traditional medicine can perhaps indicate a long association of a plant with human culture. In our survey of the medicinal uses of O. corniculata we discovered a total of 182 publications containing 892 remedies for ailments at different locations. It is evident from this map that Asia is at the centre of where O. corniculata is used medicinally (Fig. 2). In conclusion, there are several high-quality paleobotanical records of O. corniculata in eastern Asia and early documentary evidence. Furthermore, there is at least one insect that feeds on O. corniculata in the continent and there is ample documentation of the use of O. corniculata in traditional Asian medicine. Therefore, there are several lines of evidence for O. corniculata having a long presence in Asia, although the association of O. corniculata with human disturbance and agriculture has probably led to its further spread within Asia. This makes it difficult to point to a more specific area of origin. However, the archaeobotanical remains from eastern China, Japan and the Philippines do point to a long history in this area. Polynesia Oxalis corniculata was present on Hawaii (1779) and Tahiti (1769) when they were explored during the scientific expeditions of Captain James Cook (St John, 1978). Indeed, archaeological excavations have found O. corniculata seed in sediment dated from between the 15 th and 17 th centuries (McCoy, 1977; Allen, 1984). Therefore, O. corniculata is either native to Polynesia or, as suggested by Ridley (1930), was introduced by Polynesian colonizers. DISCUSSION The concept of native range is fluid and difficult to define precisely. It is hard to separate human activities from the supposed “ natural ” distribution of the organism, particularly when the term is used with agricultural weeds. Rather than define nativeness precisely we have merely tried to seek evidence for the presence of O. corniculata during its global colonization. Vaio et al. (2013) provides us with a fixed origin for the section Corniculatae in South America, but the further speciation in Australasia and North America indicates that dispersal of the section to those continents occurred hundreds of thousands of years before present. Heibl & Renner (2012) estimate that O. corniculata diverged from North American taxa (O. stricta and O. dillenii) about 10 million years ago. Therefore, there is no reason to presuppose that O. corniculata s. s. evolved in South America. Furthermore, additional resolution of the phylogeny of the section Corniculatae is needed. Vaio et al. (2013) did not include any of the known Australasian, Central American and Caribbean Corniculatae and only two of the North American species. Although Europe has often been suggested as the origin of O. corniculata, there is no convincing evidence for its occurrence here earlier than the Renaissance. In contrast, there is clear evidence of O. corniculata in eastern Asia occurring for several thousand years. Archaeological evidence supports this conclusion. Furthermore, early specimens, literature and anthropogenic usage are consistent. This conclusion also makes sense regarding the proximity of its nearest relatives. These are native to Australasia, North America and South America. This means, if one accepts the eastern Asian origin of O. corniculata, that then Oxalis section Corniculatae has a largely circum-Pacific distribution, which we find rather more parsimonious than one member of the section naturally occurring in relative isolation in Europe. There is undoubtedly considerable bias in the publication of ethnobotanical literature and in the intensity of collection of specimens from different parts of the world. In general, native plants are more often used in traditional medicines, but that is probably because there are more available (De Medeiros, Ladio & Albuquerque, 2013). Plants are also specifically introduced for their medicinal properties. Nevertheless, these independent sources of evidence both focus on subtropical eastern Asia as an important region for O. corniculata. Although this evidence is weak these results are consistent with a native origin of O. corniculata in Asia and there is little support for an origin elsewhere. The spread of O. corniculata into the Pacific islands with Polynesian voyagers is also consistent with an east Asian origin. Mitochondrial DNA and Y chromosome studies of Polynesians traces their origins back to Taiwan, the Philippines and Melanesia (Kayser et al., 2006; Ohashi et al., 2006). Indeed, a proposed spread of O. corniculata out of east Asia into the Pacific islands with early colonists could parallel the intentional introduction of Colocasia esculenta (L.) Schott (taro) to these island (Helmkampf et al., 2017). This, however, does not exclude natural dispersal between islands (Aoyama, Kawakami & Chiba, 2012). Eiten (1963) suggested Australasia and south – east Asia as the area of origin of O. corniculata. However, he did so for the wrong reasons. He supposed that the variability and broad geographic spread of O. corniculata indicated that it was parental to the whole group, rather than a more newly derived species as Vaio et al. (2013) have subsequently shown it to be. He also lumped all Australasian specimens into O. corniculata s. l. Oxalis corniculata could have been an early introduction to Europe from the east. It may have occurred in Europe before the modern discovery of the New World. An obvious pathway would have been trade along the silk route, which has been suggested as a route of introduction for crops, weeds and plant diseases in both directions (Wei et al., 2008; Smith et al., 2014). We accept the presence of O. corniculata in Europe in the 15 th century, but it might have arrived earlier, during the period of the Pax Mongolica (13 th – 14 th centuries) and the reopening of the silk routes at this time. However, the possible earlier presence of O. corniculata in Carthage indicates an earlier introduction to the Mediterranean Basin. It is interesting that peach stones also occur in the Carthage deposits. Peaches (Prunus persica (L.) Batsch) were introduced to Europe by the 1 st century AD and peaches also have an Asian origin (Faust & Timon, 1995). Coincidentally, it has also recently been suggested that taro was introduced to Europe in the 4 th century AD from south – eastern Asia (Grimaldi et al., 2018). Therefore, there are other examples of plants that have been transported between Asia and Europe for agriculture even before the 2 nd millennium. The situation in sub-Saharan Africa is still unclear and may only be resolved once the taxonomic position of O. radicosa and O. procumbens has been established. In the case of South Africa, Cape Town was originally founded 1652 and was an important trading port for the Dutch East India Company during the 17 th and 18 th century. As such, it is not hard to envisage that O. corniculata was introduced there. Nevertheless, the testimony of Floras indicates that even if O. corniculata was present in Africa before the modern era, it has become more common and today has a wider distribution than previously. In South America the final difficulty is the status of O. calachaccensis, which is indistinguishable morphologically and genetically from O. corniculata. Its presence in Bolivia suggests that O. corniculata is either native to South America, or that the specimens described as O. calachaccensis are in fact introduced O. corniculata. Certainly, Knuth described O. calachaccensis from plants collected comparatively recently (Knuth, 1915). We are inclined to think that specimens described as O. calachaccensis are modern introductions and that O. calachaccensis should be considered a synonym of O. corniculata. Relying upon physical evidence to determine the native origins of plant has its limitations and biases. A complementary method would be to examine the genetic diversity of O. corniculata globally. Taxa tend to be most genetically diverse in the area of their origin (Sakai et al., 2001) and such an approach has been useful in understanding the invasion biology of O. pes-caprae L. (Ferrero et al., 2015). It is hoped that such a study might be possible in the future if the difficulties of collecting representative populations from six continents can be surmounted. Such a study would further define the evolution and migration of this taxon worldwide.

Europe (WGSRPD:1)